This research presents a computer literacy assessment instrument to evaluate the current levels of computer literacy of students enrolled in agricultural courses at a major land grant university. Differences in levels of computer literacy resulting due to the classification of students and their majors in the College of Agriculture were measured. Stratification of general computer literacy is presented for categories of computer hardware. computer software, computer programming, agriculture computer use, and technical computer skill. Finally, the experience level of agricultural students with six specific computer software packages is presented.